Immunological evidence that HMG-CoA reductase kinase-A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases

FEBS Lett. 1995 Dec 18;377(2):189-92. doi: 10.1016/0014-5793(95)01343-1.

Abstract

Three different antibodies against the RKIN1 and BKIN12 gene products from rye and barley recognized the 58 kDa subunit of HMG-CoA reductase kinase-A (HRK-A) from Brassica oleracea on Western blots. HRK-A was also detected by an antipeptide antibody in enzyme-linked immunoassays, and this was competed by the peptide antigen. HRK-A was not recognized by antibodies against plant, mammalian and Saccharomyces cerevisiae relatives of RKIN1, i.e. wheat PKABA1, rat AMP-activated protein kinase and S. cerevisiae Snf1p. RKIN1/HMG-CoA reductase kinase-A are now among the first protein kinases in plants to be well characterized at both the molecular and biochemical levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AMP-Activated Protein Kinases
  • Amino Acid Sequence
  • Animals
  • Antibodies / immunology
  • Brassica / enzymology*
  • Cross Reactions
  • Fungal Proteins / immunology
  • Molecular Sequence Data
  • Multienzyme Complexes / chemistry*
  • Multienzyme Complexes / immunology
  • Peptides / immunology
  • Plant Proteins / chemistry*
  • Plant Proteins / immunology
  • Protein Kinases / chemistry*
  • Protein Kinases / immunology
  • Protein-Serine-Threonine Kinases / chemistry*
  • Protein-Serine-Threonine Kinases / immunology
  • Rats

Substances

  • Antibodies
  • Fungal Proteins
  • Multienzyme Complexes
  • Peptides
  • Plant Proteins
  • Protein Kinases
  • BKIN12 protein, Hordeum vulgare
  • RKIN1 protein, Secale cereale
  • Protein-Serine-Threonine Kinases
  • AMP-Activated Protein Kinases