Effects of myosin light chain kinase inhibitors on delayed rectifier potassium current in bullfrog sympathetic neurons

Neurosci Lett. 1995 Sep 1;197(1):75-7. doi: 10.1016/0304-3940(95)11884-y.

Abstract

Actions of myosin light chain kinase inhibitors were tested on delayed rectifier potassium current (IK) in dissociated bullfrog sympathetic neurons. A microbial product, wortmannin (10 microM, extracellularly) and a synthetic peptide, SM-1 (20 microM, intracellularly) caused approximately 35 mV hyperpolarizing shift of the inactivation curve. Substitution of ATP (1.15 mM) in the pipette solution with 5'-adenylylimidodiphosphate mimicked the actions of wortmannin and SM-1. Results suggest that phosphorylation of myosin may modulate kinetics for the inactivation of IK.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Imidodiphosphate / pharmacology
  • Androstadienes / pharmacology
  • Animals
  • Electrophysiology
  • Enzyme Inhibitors / pharmacology
  • Ganglia, Sympathetic / cytology
  • Ganglia, Sympathetic / drug effects
  • In Vitro Techniques
  • Myosin-Light-Chain Kinase / antagonists & inhibitors*
  • Myosin-Light-Chain Kinase / pharmacology
  • Neurons / drug effects
  • Neurons / metabolism*
  • Peptide Fragments / pharmacology
  • Potassium Channels / drug effects
  • Potassium Channels / metabolism*
  • Rana catesbeiana
  • Sympathetic Nervous System / cytology
  • Sympathetic Nervous System / drug effects
  • Sympathetic Nervous System / metabolism*
  • Wortmannin

Substances

  • Androstadienes
  • Enzyme Inhibitors
  • Peptide Fragments
  • Potassium Channels
  • SM 1 peptide
  • Adenylyl Imidodiphosphate
  • Myosin-Light-Chain Kinase
  • Wortmannin