Overexpression of human p21waf1/cip1 arrests the growth of chicken embryo fibroblasts transformed by individual oncogenes

Oncogene. 1995 Dec 21;11(12):2609-18.

Abstract

In normal cells, cell growth and division is controlled by the interplay between proto-oncogenes and tumor suppressor genes. Cancer cells usually have both activated an oncogene and have lost a functional tumor suppressor gene. High level expression of a tumor suppressor, p53, can block the growth of cancer cells. waf1/cip1 is transactivated by the tumor suppressor p53 and the p21waf1/cip1 protein is itself a suppressor of cell growth. To test the effect of growth suppression genes on the growth of cells transformed by individual oncogenes, we have used replication-competent retroviral vectors to induce high level expression of p53 and p21waf1/cip1. Overexpression of p21waf1/cip1 arrests the growth of chicken embryo fibroblasts (CEF) transformed by v-Src, tf-Ras, c-Mos and c-Myc. These data suggest that p21waf1/cip1 might be a useful tool in gene therapy for human cancer.

MeSH terms

  • Animals
  • Cell Division
  • Cell Transformation, Neoplastic / pathology*
  • Chick Embryo
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclins / physiology*
  • Enzyme Inhibitors*
  • G1 Phase
  • Humans
  • Oncogenes*
  • Rabbits
  • Tumor Suppressor Protein p53 / analysis

Substances

  • CDKN1A protein, human
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclins
  • Enzyme Inhibitors
  • Tumor Suppressor Protein p53