[The use of the immunoperoxidase test with monoclonal antibodies for detecting influenza A viruses]

Mikrobiol Z. 1995 Jul-Aug;57(4):54-9.
[Article in Russian]

Abstract

The immunoperoxidase test was used to detect influenza virus in cells of a chorionallanthois shell of infected chicken embryos. Application of monoclonal antibodies D8 and A11 in the analysis has permitted detecting reproduction of type A (subtypes H1N1, H2N2, H3N2) viruses, the PGA titre of the respective allantois liquids being not lower than 1:16. The matrix protein and hemagglutinin, detection of which underlies this analysis, were found on the cell membrane, in the perinucleus region and as cytoplasmic inclusions.

Publication types

  • English Abstract

MeSH terms

  • Allantois / immunology
  • Allantois / virology
  • Animals
  • Antibodies, Monoclonal*
  • Antigens, Viral / analysis
  • Chick Embryo
  • Chorion / immunology
  • Chorion / virology
  • Hemagglutinins, Viral / immunology
  • Humans
  • Hybridomas / immunology
  • Immunoenzyme Techniques*
  • Influenza A virus / immunology*
  • Viral Matrix Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • Antigens, Viral
  • Hemagglutinins, Viral
  • M-protein, influenza virus
  • Viral Matrix Proteins