Keratinophilic fungi were isolated from samples of soil collected in the extremity of Chile, in Southern Shetland and Antarctic continent. Two new species, Microsporum magellanicum and Cunninghamella antarctica are described. Microsporum magellanicum is characterized by verrucose or verruculose clavate macroconidia predominantly uniseptate, 4-87-2micronx14-4-21-6micron. Cunninghamella antarctica has conidiophores usually verticillately, pseudoverticillately and sympodially branched; and globose conidia with evident spines, 12-8-16micron in diameter.