The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins

Cell. 1995 Dec 29;83(7):1243-52. doi: 10.1016/0092-8674(95)90149-3.

Abstract

The 75 kDa tumor necrosis factor receptor (TNFR2) transduces extracellular signals via receptor-associated cytoplasmic proteins. Two of these signal transducers, TRAF1 and TRAF2, were isolated and characterized previously. We report here the biochemical purification and subsequent molecular cloning of two novel TNFR2-associated proteins, designated c-IAP1 and c-IAP2, that are closely related mammalian members of the inhibitor of apoptosis protein (IAP) family originally identified in baculoviruses. The viral and cellular IAPs contain N-terminal baculovirus IAP repeat (BIR) motifs and a C-terminal RING finger. The c-IAPs do not directly contact TNFR2, but rather associate with TRAF1 and TRAF2 through their N-terminal BIR motif-comprising domain. The recruitment of c-IAP1 or c-IAP2 to the TNFR2 signaling complex requires a TRAF2-TRAF1 heterocomplex.

MeSH terms

  • Animals
  • Apoptosis / physiology
  • Cell Cycle Proteins / physiology
  • Cell Line / cytology
  • Cell Line / physiology
  • Cloning, Molecular
  • Humans
  • Inhibitor of Apoptosis Proteins
  • Kidney / cytology
  • Kidney / physiology
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Proteins / physiology*
  • RNA, Messenger / analysis
  • Receptors, Tumor Necrosis Factor / isolation & purification
  • Receptors, Tumor Necrosis Factor / physiology*
  • Sequence Homology, Amino Acid
  • Signal Transduction / physiology
  • T-Lymphocytes, Cytotoxic / cytology
  • T-Lymphocytes, Cytotoxic / physiology
  • TNF Receptor-Associated Factor 1
  • TNF Receptor-Associated Factor 2
  • Ubiquitin-Protein Ligases
  • Viral Proteins / physiology*

Substances

  • Cell Cycle Proteins
  • Inhibitor of Apoptosis Proteins
  • Proteins
  • RNA, Messenger
  • Receptors, Tumor Necrosis Factor
  • TNF Receptor-Associated Factor 1
  • TNF Receptor-Associated Factor 2
  • Viral Proteins
  • inhibitor of apoptosis, Nucleopolyhedrovirus
  • BIRC2 protein, human
  • Birc2 protein, mouse
  • Ubiquitin-Protein Ligases

Associated data

  • GENBANK/L49431
  • GENBANK/L49432
  • GENBANK/L49433