Identification and expression cloning of a leptin receptor, OB-R

Cell. 1995 Dec 29;83(7):1263-71. doi: 10.1016/0092-8674(95)90151-5.

Abstract

The ob gene product, leptin, is an important circulating signal for the regulation of body weight. To identify high affinity leptin-binding sites, we generated a series of leptin-alkaline phosphatase (AP) fusion proteins as well as [125I]leptin. After a binding survey of cell lines and tissues, we identified leptin-binding sites in the mouse choroid plexus. A cDNA expression library was prepared from mouse choroid plexus and screened with a leptin-AP fusion protein to identify a leptin receptor (OB-R). OB-R is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Genetic mapping of the gene encoding OB-R shows that it is within the 5.1 cM interval of mouse chromosome 4 that contains the db locus.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites / physiology
  • Choroid Plexus / physiology
  • Choroid Plexus / ultrastructure
  • Chromosome Mapping
  • Cloning, Molecular
  • Gene Expression / physiology
  • Humans
  • Leptin
  • Mice
  • Mice, Inbred C57BL
  • Mice, Obese
  • Molecular Sequence Data
  • Obesity / genetics*
  • Obesity / metabolism
  • Proteins / genetics*
  • Proteins / isolation & purification
  • Proteins / metabolism
  • RNA, Messenger / analysis
  • Receptors, Cell Surface / isolation & purification
  • Receptors, Leptin

Substances

  • Leptin
  • Proteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Leptin
  • leptin receptor, human
  • leptin receptor, mouse

Associated data

  • GENBANK/U42467
  • GENBANK/U43168