2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region

Cell. 1996 Jan 12;84(1):155-64. doi: 10.1016/s0092-8674(00)81002-8.


We have determined the 2.0 A crystal structure of a fragment of human fibronectin encompassing the seventh through the RGD-containing tenth type III repeats (FN7-10). The structure reveals an extended rod-like molecule with a long axis of approximately 140 A and highly variable relationships between adjacent domains. An unusually small rotation between domains 9 and 10 creates a distinctive binding site, in which the RGD loop from domain 10 and the "synergy" region from domain 9 are on the same face of FN7-10 and thus easily accessible to a single integrin molecule. The cell-binding RGD loop is well-ordered in this structure and extends approximately 10 A away from the FN7-10 core.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Crystallography
  • Fibronectins / chemistry*
  • Fibronectins / ultrastructure
  • Humans
  • Image Processing, Computer-Assisted
  • Molecular Sequence Data
  • Oligopeptides / chemistry*
  • Protein Conformation


  • Fibronectins
  • Oligopeptides
  • arginyl-glycyl-aspartic acid