Separation of sister chromatids in mitosis requires the Drosophila pimples product, a protein degraded after the metaphase/anaphase transition

Cell. 1996 Jan 12;84(1):25-35. doi: 10.1016/s0092-8674(00)80990-3.


Mutations in the Drosophila genes pimples and three rows result in a defect of sister chromatid separation during mitosis. As a consequence, cytokinesis is also defective. However, cell cycle progression including the mitotic degradation of cyclins A and B is not blocked by the failure of sister chromatid separation, and as a result, metaphase chromosomes with twice the normal number of chromosome arms still connected in the centromeric region are observed in the following mitosis, pimples encodes a novel protein that is rapidly degraded in mitosis. Our observations suggest that Pimples and Three rows act during mitosis to release the cohesion between sister centromeres.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anaphase / genetics*
  • Animals
  • Antigens, Protozoan*
  • Base Sequence
  • Blotting, Northern
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / metabolism
  • Cell Division / genetics
  • Centromere / genetics
  • Centromere / metabolism
  • Chromatids / genetics*
  • Chromosomes / genetics
  • Chromosomes / metabolism
  • Cloning, Molecular
  • Cyclins / metabolism
  • Drosophila / genetics*
  • Gene Expression Regulation, Developmental / genetics
  • Genes, Insect / physiology
  • In Situ Hybridization, Fluorescence
  • Larva / genetics
  • Metaphase / genetics*
  • Mitosis / genetics
  • Molecular Sequence Data
  • Mutation / physiology
  • Phenotype
  • Protozoan Proteins / genetics


  • Antigens, Protozoan
  • Cell Cycle Proteins
  • Cyclins
  • Protozoan Proteins
  • antigen PIM, Theileria parva

Associated data

  • GENBANK/X93653