Cytokinesis in the Arabidopsis embryo involves the syntaxin-related KNOLLE gene product

Cell. 1996 Jan 12;84(1):61-71. doi: 10.1016/s0092-8674(00)80993-9.

Abstract

The embryo of the flowering plant Arabidopsis develops by a regular pattern of cell divisions and cell shape changes. Mutations in the KNOLLE (KN) gene affect the rate and plane of cell divisions as well as cell morphology, resulting in mutant seedlings with a disturbed radial organization of tissue layers. At the cellular level, mutant embryos are characterized by incomplete cross walls and enlarged cells with polyploid nuclei. The KN gene was isolated by positional cloning. The predicted KN protein has similarity to syntaxins, a protein family involved in vesicular trafficking. During embryogenesis, KN transcripts are detected in patches of single cells or small cell groups. Our results suggest a function for KN in cytokinesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / embryology*
  • Arabidopsis Proteins*
  • Base Sequence
  • Cell Division / genetics
  • Cell Division / physiology
  • Cloning, Molecular
  • Gene Expression Regulation, Plant / physiology
  • Genes, Plant / physiology
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Mutation / physiology
  • Qa-SNARE Proteins
  • Seeds / genetics
  • Sequence Homology, Amino Acid
  • Transcription, Genetic / physiology

Substances

  • Arabidopsis Proteins
  • KNOLLE protein, Arabidopsis
  • Membrane Proteins
  • Qa-SNARE Proteins

Associated data

  • GENBANK/U39451
  • GENBANK/U39452