E-cadherin and OB-cadherin mRNA levels in normal human colon and colon carcinoma

Exp Mol Pathol. 1995 Apr;62(2):118-22. doi: 10.1006/exmp.1995.1013.

Abstract

A polymerase chain reaction strategy was utilized to identify members of the cadherin family of cell adhesion molecules that are expressed in normal human colon and colon carcinoma. E-cadherin (an epithelial cadherin) and OB-cadherin (a mesenchymal cadherin) were found to be present in the colon specimens. Furthermore, a novel semiquantitative polymerase chain reaction (PCR) assay was developed for each of these two cadherins. These assays were used to determine the relative levels of E-cadherin and OB-cadherin in normal human colon and colon carcinoma specimens. E-cadherin levels were found to be reduced approximately twofold in the adenocarcinoma specimens in comparison to matched normal colon specimens. In contrast, OB-cadherin levels did not correlate with malignant transformation. We speculate that this novel PCR method will be widely applicable for assessing E-cadherin mRNA levels in carcinomas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / chemistry*
  • Base Sequence
  • Cadherins / analysis*
  • Cadherins / genetics
  • Colon / chemistry*
  • Colonic Neoplasms / chemistry*
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Sensitivity and Specificity

Substances

  • Cadherins
  • RNA, Messenger
  • osteoblast cadherin

Associated data

  • GENBANK/J00423
  • GENBANK/L34056
  • GENBANK/L34545