Molecular analysis of the sheep cathelin family reveals a novel antimicrobial peptide

FEBS Lett. 1995 Dec 27;377(3):519-22. doi: 10.1016/0014-5793(95)01390-3.

Abstract

Cathelin-related genes are characterized by the presence of a prepro sequence which is highly conserved both within and between species. 3' RACE analysis on sheep bone marrow RNA, using a primer based on a conserved cathelin family coding region, demonstrated the presence of at least three ovine cathelin-related cDNAs. One of these encodes a novel prepropeptide with a predicted C-terminal cleavage product RGLRRLGRKIAHG-VKKYGPTVLRIIRIAG. The chemically synthesized form of this 29 amino acid peptide is shown to be a thermostable, broad spectrum, bactericidal agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anti-Infective Agents / pharmacology*
  • Antimicrobial Cationic Peptides*
  • Base Sequence
  • Bone Marrow / chemistry*
  • Dose-Response Relationship, Drug
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Multigene Family*
  • Peptides / genetics*
  • Peptides / pharmacology
  • Protein Precursors
  • Proteins / genetics*
  • Proteins / pharmacology
  • Sequence Homology, Amino Acid
  • Sheep

Substances

  • Anti-Infective Agents
  • Antimicrobial Cationic Peptides
  • Peptides
  • Protein Precursors
  • Proteins
  • SC5 synthetic antimicrobial peptide
  • cathelin

Associated data

  • GENBANK/X92757
  • GENBANK/X92758