Maleylacetate reductases in chloroaromatic-degrading bacteria using the modified ortho pathway: comparison of catalytic properties

J Bacteriol. 1996 Jan;178(1):298-300. doi: 10.1128/jb.178.1.298-300.1996.

Abstract

The maleylacetate reductases from Pseudomonas aeruginosa RHO1 and Alcaligenes eutrophus JMP134 were tested for activity and affinity to various maleylacetates as well as dechlorinating properties. The dechlorinating activity and the kcat/Km values revealed high-level similarity of these reductases to that of Pseudomonas sp. strain B13.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcaligenes / enzymology*
  • Biodegradation, Environmental
  • Kinetics
  • Maleates / metabolism
  • Models, Chemical
  • NAD / metabolism
  • Oxidoreductases / metabolism*
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Pseudomonas aeruginosa / enzymology*
  • Substrate Specificity

Substances

  • Maleates
  • NAD
  • maleoylacetic acid
  • Oxidoreductases
  • Oxidoreductases Acting on CH-CH Group Donors
  • maleylacetate reductase