Differentially transcribed regions of Haloferax volcanii genome depending on the medium salinity

J Bacteriol. 1996 Jan;178(1):309-13. doi: 10.1128/jb.178.1.309-313.1996.


To identify genomic regions involved in osmoregulation in the extremely halophilic archaeon Haloferax volcanii, we used a technique which involves hybridization of cDNAs obtained at different salinities against a cosmid library of the organism. Both low and high salt concentrations trigger differential expression; however, adaptation to low salinities seems to elicit a wider response. The presence of a large domain within the largest of the megaplasmids with a strong response to low salt concentrations is noteworthy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Blotting, Southern
  • Chromosome Mapping
  • Culture Media
  • DNA, Complementary
  • Gene Expression Regulation, Bacterial / drug effects*
  • Genome, Bacterial
  • Halobacteriaceae / genetics*
  • Sodium Chloride / pharmacology*
  • Transcription, Genetic / drug effects*
  • Water-Electrolyte Balance


  • Culture Media
  • DNA, Complementary
  • Sodium Chloride