T cell antigenic and neuritogenic activity of recombinant human peripheral myelin P2 protein

J Neuroimmunol. 1995 Dec 31;63(2):149-56. doi: 10.1016/0165-5728(95)00139-5.


The major neuritogenic protein of peripheral nerve myelin is the P2 protein. Human P2 is a candidate autoantigen in inflammatory demyelinating diseases of the peripheral nervous system. Since human P2 is not readily available, we produced full-length recombinant human P2 protein (rhP2) in Escherichia coli. RhP2 was recognized by neuritogenic rat T cell lines and induced experimental autoimmune neuritis in Lewis rats. Production of rhP2 allowed the generation of human T cell lines reactive to the autologous protein. Studies of human T cell autoreactivity as well as efforts to use hP2 as a tolerogen will be facilitated by the large-scale expression of rhP2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Autoantibodies
  • Base Sequence
  • Escherichia coli / genetics
  • Female
  • Humans
  • Immunization
  • Molecular Sequence Data
  • Myelin P2 Protein / immunology*
  • Myelin P2 Protein / isolation & purification
  • Neuritis / immunology
  • Peripheral Nervous System / immunology
  • Rats
  • Rats, Inbred Lew
  • Recombinant Proteins / immunology
  • T-Lymphocytes / immunology*


  • Autoantibodies
  • Myelin P2 Protein
  • Recombinant Proteins