A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors

J Exp Med. 1996 Jan 1;183(1):187-94. doi: 10.1084/jem.183.1.187.


Bone marrow of both normal and rearrangement-deficient mice contains a small population of B220(CD45R)+ cells, which do not express the B lineage marker CD19. Instead, part of this population coexpresses the surface marker CD43 and lacks or expresses very low levels of heat stable antigen (HSA) and BP-1, thus representing a part of Hardy's fraction A (B220(+)-CD43+HSA-, BP-1-) of B lineage development. However, some 20-40% of these B220(+)-CD19- cells also coexpress the NK1.1 surface molecule and do not express genes like VpreB or B29 restricted to the B cell lineage. These cells respond to recombinant interleukin 2 in vitro, and develop into killer cells that can lyse the prototypic NK target tumor cell, YAC-1, as well as syngeneic normal lipopolysaccharide or concanavalin A blasts, providing they lack the surface expression of major histocompatibility complex class I molecules. The implications of these findings for studies on B lymphopoiesis are discussed. It is suggested that the CD19-specific monoclonal antibody is more reliable, as in humans, than B220(CD45R) to detect B lineage cells in mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, CD19 / analysis*
  • Antigens, CD19 / immunology
  • Base Sequence
  • Bone Marrow Cells*
  • Chromium / metabolism
  • Clone Cells
  • Cytotoxicity, Immunologic
  • Female
  • Flow Cytometry
  • Hematopoietic Stem Cells / chemistry*
  • Hematopoietic Stem Cells / drug effects
  • Hematopoietic Stem Cells / immunology
  • Interleukin-2 / pharmacology
  • Killer Cells, Lymphokine-Activated
  • Killer Cells, Natural / chemistry*
  • Killer Cells, Natural / drug effects
  • Killer Cells, Natural / immunology
  • Leukocyte Common Antigens / analysis*
  • Lymphocyte Subsets / chemistry*
  • Lymphocyte Subsets / drug effects
  • Lymphocyte Subsets / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Polymerase Chain Reaction


  • Antibodies, Monoclonal
  • Antigens, CD19
  • Interleukin-2
  • Chromium
  • Leukocyte Common Antigens