Plasmid coding for drug resistance and invasion of epithelial cells in enteropathogenic Escherichia coli 0111:H

Microb Pathog. 1995 Jun;18(6):387-99. doi: 10.1006/mpat.1995.0035.


Enteropathogenic Escherichia coli (EPEC) can adhere to, invade and multiply in human epithelial cells. To define the elements required for bacterial invasion, we isolated from an 0111:H- EPEC a 6.6 kb plasmid that is capable of conferring to an avirulent, non-adherent E. coli K12 strain (DK1) the capacity to invade epithelial cells. With this system a dissociation was possible between bacterial invasion and adherence to epithelial cells. Bacteria containing this plasmid synthesise a protein of 32 kDa (pl 4.93) which seemed to be required for cell invasion. The results provide a new basis for strategies to prevent EPEC infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Adhesion
  • Chromosome Mapping
  • Epithelial Cells
  • Epithelium / microbiology
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Escherichia coli / pathogenicity
  • HeLa Cells
  • Humans
  • Kanamycin Resistance / genetics
  • Nucleic Acid Hybridization
  • Plasmids*
  • Rabbits
  • Tumor Cells, Cultured
  • Virulence / genetics