Combined mapping of AFLP and RFLP markers in barley

Mol Gen Genet. 1995 Nov 1;249(1):65-73. doi: 10.1007/BF00290237.

Abstract

AFLP marker technology allows efficient DNA fingerprinting and the analysis of large numbers of polymorphic restriction fragments on polyacrylamide gels. Using the doubled haploids from the F1 of the cross Proctor x Nudinka, 118 AFLP markers were mapped onto a barley (Hordeum vulgare L.) RFLP map, also including five microsatellite and four protein marker loci. The AFLP markers mapped to all parts of the barley chromosomes and filled in the gaps on barley chromosomes 2L, 4L and 6 in which no RFLP loci had been mapped. Interestingly, the AFLP markers seldom interrupted RFLP clusters, but grouped next to them. The combined map covers 1873 cM, with a total of 282 markers. The merging of AFLP and RFLP markers increased the total map length; 402 cM were added to the map at the tips of chromosomes or in regions corresponding to earlier gaps. Another 375 cM resulted from mapping AFLP markers near to RFLP clusters or in between non-clustered RFLP markers.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Mapping*
  • Crosses, Genetic
  • DNA Fingerprinting
  • DNA Primers
  • DNA, Plant / genetics
  • DNA, Plant / isolation & purification
  • Genetic Markers
  • Genome, Plant
  • Haploidy
  • Hordeum / genetics*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length*
  • Restriction Mapping

Substances

  • DNA Primers
  • DNA, Plant
  • Genetic Markers
  • Oligodeoxyribonucleotides