DOGR1 and DOGR2: two genes from Saccharomyces cerevisiae that confer 2-deoxyglucose resistance when overexpressed

Yeast. 1995 Oct;11(13):1233-40. doi: 10.1002/yea.320111303.


Saccharomyces cerevisiae contains two genes (DOGR1 and DOGR2) that are able to confer 2-deoxyglucose resistance when they are overexpressed. These genes are very similar, sharing 92% identity at the protein level. They code for two isoenzymes with 2-deoxyglucose-6 phosphate (2-DOG-6P) phosphatase activity. These enzymes have been purified and characterized. DogR1p shows an optimum pH of 6, an optimum temperature of 30 degrees C and a KM on 2-DOG-6P of 17 mM. DogR2p shows a similar optimum pH, but the optimum temperature is 40 degrees C and it exhibits a KM on 2-DOG-6P of 41 mM. Both enzymes require 10 mM-MgCl2 for maximal activity and they are inhibited by inorganic phosphate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Base Sequence
  • Deoxyglucose / toxicity*
  • Genes, Fungal*
  • Glucose-6-Phosphate* / analogs & derivatives*
  • Glucosephosphates / metabolism
  • Molecular Sequence Data
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*


  • Glucosephosphates
  • 2-deoxyglucose-6-phosphate
  • Glucose-6-Phosphate
  • Deoxyglucose

Associated data

  • GENBANK/U00062
  • GENBANK/U03107