Genetic heterogeneity in Niemann-Pick C disease: a study using somatic cell hybridization and linkage analysis

Am J Hum Genet. 1996 Jan;58(1):118-25.


The primary molecular defect underlying Niemann-Pick C disease (NPC) is still unknown. A wide spectrum of clinical and biochemical phenotypes has previously been documented. Indication of genetic heterogeneity has recently been provided for one patient. In the present study, somatic cell hybridization experiments were carried out on skin fibroblast cultures from 32 unrelated NPC patients covering the range of known clinical and biochemical phenotypes. The criterion for complementation was the restoration of a normal intracellular fluorescent pattern in polykaryons stained with filipin to document cholesterol distribution. Crosses between the various cell lines revealed a major complementation group comprising 27 unrelated patients and a second minor group comprising 5 patients. Linkage analysis in one multiplex family belonging to the minor complementation group showed that the mutated gene does not map to the 18q11-12 region assigned to the major gene. Patients in the first group spanned the whole spectrum of clinical and cellular phenotypes. No consistent clinical or biochemical phenotypes was associated with the second complementation group. Three of the five group 2 patients, however, presented with a new rare phenotype associated with severe pulmonary involvement leading to death within the first year of life. No biochemical abnormality specific of either group could be demonstrated with regard to tissue lipid storage pattern, intralysosomal cholesterol storage, and regulation of cholesterol homeostasis. Mutations affecting at least two different genes have thus been shown to underlie NPC. The two gene products may function together or sequentially in a common metabolic pathway affecting intracellular cholesterol transport.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Age Factors
  • Cells, Cultured
  • Child
  • Child, Preschool
  • Cholesterol / metabolism
  • Chromosome Mapping
  • Chromosomes, Human, Pair 18*
  • Female
  • Fetus
  • Fibroblasts / metabolism
  • Filipin
  • Genetic Complementation Test
  • Genetic Linkage
  • Humans
  • Hybrid Cells
  • Infant
  • Liver / metabolism
  • Niemann-Pick Diseases / genetics*
  • Niemann-Pick Diseases / mortality
  • Phenotype
  • Phospholipids / metabolism
  • Polymerase Chain Reaction
  • Pregnancy
  • Skin / metabolism
  • Sphingomyelins / metabolism
  • Spleen / metabolism


  • Phospholipids
  • Sphingomyelins
  • Filipin
  • Cholesterol