Involvement of Wnt-1 in the formation of the mes/metencephalic boundary

Mech Dev. 1995 Sep;53(1):23-34. doi: 10.1016/0925-4773(95)00421-1.


Wnt-1, a putative signaling molecule, is required before the 7 somite stage (E8.5) for the development of midbrain structures in the mouse. We show here that Wnt-1 is also needed for the formation of a boundary between the mesencephalic and metencephalic domains of the neural tube. In embryos homozygous for the Wnt-1sw allele, mesencephalic and metencephalic markers fail to segregate and the establishment of a straight limit of Otx-2 and Wnt-1 expression at the mid-hindbrain junction is impaired. In addition, as observed previously in heterotopic mes/metencephalic transplantation experiments in avian embryos, Wnt-1 expression is induced at the border of ectopic mes- and metencephalic islands observed in Wnt-1sw/sw mutants, suggesting that, in situ, interactions between mes- and metencephalic cells reinforce Wnt-1 expression at the boundary.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cerebellum / embryology
  • Choroid Plexus / embryology
  • Drosophila / embryology
  • Embryonic and Fetal Development / physiology
  • Gestational Age
  • Homozygote
  • Mesencephalon / embryology*
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Phenotype
  • Pons / embryology*
  • Proto-Oncogene Proteins / physiology*
  • Wnt Proteins
  • Wnt1 Protein
  • Zebrafish Proteins*


  • Proto-Oncogene Proteins
  • Wnt Proteins
  • Wnt1 Protein
  • Wnt1 protein, mouse
  • Zebrafish Proteins