Cytokine (IL-10, IL-6) induction of tissue inhibitor of metalloproteinase 1 in primary human prostate tumor cell lines

Oncol Res. 1995;7(3-4):173-81.


Northern blots and scintillation counting showed that tissue inhibitor of metalloproteinase 1 (TIMP-1) mRNA was expressed by low passage, primary epithelial cultures (n = 5) of low-grade human prostatic carcinoma. TIMP-1 mRNA levels were normally low in the primary cell lines, but were inducible by interleukin (IL) 10 and 6. Dose and time-course studies indicated that IL-10 was the most potent stimulator of TIMP-1 expression. Cycloheximide blocked the effects of IL-10 in a reversible manner. In situ hybridization assays with TIMP-1 oligonucleotide antisense probes confirmed the northern blot results and indicated that IL-10 preferentially stimulated TIMP-1 mRNA synthesis. We suggest that IL-10, and to a lesser extent IL-6, may normally influence TIMP-1 expression by human prostatic epithelial cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Northern
  • Epithelium / metabolism
  • Glycoproteins / biosynthesis*
  • Glycoproteins / genetics
  • Humans
  • In Situ Hybridization
  • Interleukin-10 / pharmacology*
  • Interleukin-6 / pharmacology*
  • Male
  • Prostatic Neoplasms / metabolism*
  • Protease Inhibitors / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Tissue Inhibitor of Metalloproteinases
  • Tumor Cells, Cultured / drug effects


  • Glycoproteins
  • Interleukin-6
  • Protease Inhibitors
  • RNA, Messenger
  • Tissue Inhibitor of Metalloproteinases
  • Interleukin-10