Stress signal, mediated by a Hog1-like MAP kinase, controls sexual development in fission yeast

FEBS Lett. 1996 Jan 15;378(3):207-12. doi: 10.1016/0014-5793(95)01442-x.

Abstract

We identified the phh1+ gene that encodes a MAP kinase as the effector of Wis1 MAP kinase kinase in fission yeast, which is highly homologous with HOG1 of S. cerevisiae. Heterothalic phh1 dsiruptant is phenotypically indistinguishable from wis1 deletion mutant, both displaying the same extent of partial sterility and enhanced sensitivity to a variety of stress. In phh1 disruptant, nitrogen starvation-induced expression of ste11+, a key controller of sexual differentiation, is markedly diminished. Ectopic expression of ste11+ effectively restores fertility, but not stress resistance, to the phh1 disruptant. These data show that stress signal, mediated by a MAP kinase, is required for efficient start of sexual differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Calcium-Calmodulin-Dependent Protein Kinases / chemistry*
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
  • Cell Division
  • Culture Media / chemistry
  • Fertility / genetics
  • Fungal Proteins / genetics
  • Gene Expression Regulation, Fungal
  • Hot Temperature
  • MAP Kinase Kinase Kinases / genetics
  • Mitogen-Activated Protein Kinase Kinases*
  • Mitogen-Activated Protein Kinases*
  • Molecular Sequence Data
  • Nitrogen / chemistry
  • Nitrogen / metabolism
  • Osmotic Pressure
  • Protein-Serine-Threonine Kinases / metabolism
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae Proteins*
  • Schizosaccharomyces / enzymology
  • Schizosaccharomyces / physiology*
  • Schizosaccharomyces pombe Proteins*
  • Sequence Homology, Amino Acid
  • Sex Differentiation*
  • Signal Transduction / genetics
  • Signal Transduction / physiology*
  • Spores
  • Stress, Physiological*
  • Transcription Factors*

Substances

  • Culture Media
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces pombe Proteins
  • Transcription Factors
  • ste11 protein, S pombe
  • Protein-Serine-Threonine Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • HOG1 protein, S cerevisiae
  • Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase Kinases
  • Ste11 protein, S cerevisiae
  • Mitogen-Activated Protein Kinase Kinases
  • wis1 protein, S pombe
  • Nitrogen