Modulation of a volume-regulated chloride current by F-actin

J Membr Biol. 1995 Oct;147(3):283-94. doi: 10.1007/BF00234526.

Abstract

We have examined whether F-actin integrity is involved in activation of a volume-regulated Cl- current (VRChlC) in B-lymphocytes. VRChlC activation was initiated in response to establishing a whole cell recording in the presence of a hyposmotic gradient. Parallel confocal microscopy experiments using Rhodamine-Phalloidin (R-P) as a specific marker of F-actin showed that the submembrane actin ring is reversibly disrupted in response to an hyposmotic gradient. Disruptions of cortical F-actin integrity by 50 microM cytochalasin B (CB) does not trigger activation of VRChlC under isosmotic conditions or potentiate the rate of activation when the osmolarity of the extracellular solution was decreased by 75%. However, incubation with CB increased the rate of VRChlC activation in response to a 90% hyposmotic gradient. Phalloidin, a stabilizer of F-actin, decreases the rate of VRChlC activation in response to a 90% gradient, but has no effect in response to a 75% gradient. These observations suggest that disassembly of cortical F-actin is not critical for VRChlC activation in B-lymphocytes. The integrity of cortical F-actin, however, can exert a modulatory effect on the rate of VRChlC activation in the presence of a hyposmotic gradient.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / metabolism*
  • Actins / ultrastructure
  • B-Lymphocytes / metabolism*
  • Chlorides / metabolism*
  • Cytochalasin B / pharmacology
  • Humans
  • Ion Transport
  • Microscopy, Confocal
  • Osmotic Pressure
  • Phalloidine / pharmacology
  • Tumor Cells, Cultured

Substances

  • Actins
  • Chlorides
  • Phalloidine
  • Cytochalasin B