Short-chain lipopolysaccharide mutants of serogroup B Neisseria meningitidis of potential value for production of outer membrane vesicle vaccines

Microb Pathog. 1995 Sep;19(3):159-68. doi: 10.1006/mpat.1995.0054.


Four lipopolysaccharide (LPS) mutants (Mu-1 to Mu-4) were isolated after exposing Neisseria meningitidis strain 44/76 to pyocins from Pseudomonas aeruginosa. Parent strain LPS contained one major SDS-PAGE band expressing the immunotype determinants of L3, L3,7 and L3,7,9 and a minor band of higher mobility expressing the immunotype determinants of L8, L8a, L1,8,10 and L11. Each mutant LPS appeared as one SDS-PAGE band of higher mobility than the bands of the parent strain. None of these LPSs expressed the immunotype determinants of the parent strain, except Mu-4 LPS which reacted with the L11-specific MAb 4C4. Strain 44/76 LPS was found to contain galactose (Gal), glucose (Glc), heptose (Hep), glucosamine (GlcN), and 2-keto-3-deoxy-octulosonic acid (Kdo) in the molar ratios of 1.9:1.3:1.7:3.5:2.1. The corresponding ratios of the mutants were: Mu-4, 0:1.7:1.7:2.8:2.0; Mu-3, 0:0:1.7:2.4:1.6; Mu-2, 0:0:2.1:1.8:2.0, Mu-1, 0:0:1.8:1.9. Thus, all mutant LPSs lacked Gal and possessed less GlcN as compared to strain 44/76 LPS. Consequently, these mutants do not express the lacto-N-neo-tetraose (Gal1-4GlcN1-3Gal1-4Glc) commonly found as a part of meningococcal LPS and also on structures of human erythrocytes. These LPS mutants will be considered for use in production of OMV vaccines without host-like antigens, which might favour induction of antibodies to more conserved epitopes of meningococcal LPS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / immunology
  • Antibodies, Monoclonal / immunology
  • Bacterial Outer Membrane Proteins / analysis
  • Bacterial Vaccines / chemistry
  • Bacterial Vaccines / genetics
  • Bacterial Vaccines / immunology
  • Carbohydrate Sequence
  • Humans
  • Lipopolysaccharides / chemistry*
  • Lipopolysaccharides / immunology
  • Molecular Sequence Data
  • Mutation
  • Neisseria meningitidis / chemistry
  • Neisseria meningitidis / genetics*
  • Neisseria meningitidis / immunology
  • Pyocins / pharmacology
  • Serotyping


  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Bacterial Outer Membrane Proteins
  • Bacterial Vaccines
  • Lipopolysaccharides
  • Pyocins
  • opc protein, bacteria