A novel PRD I and TG binding activity involved in virus-induced transcription of IFN-A genes

Nucleic Acids Res. 1995 Dec 25;23(24):5055-63. doi: 10.1093/nar/23.24.5055.

Abstract

Comparative analysis of the inducible elements of the mouse interferon A4 and A11 gene promoters (IE-A4 and IE-A11) by transient transfection experiments, DNase 1 footprinting and electrophoretic mobility shift assays resulted in identification of a virus-induced binding activity suggested to be involved in NDV-induced activation of transcription of these genes. The virus-induced factor, termed VIF, is activated early by contact of virions with cells. It specifically recognizes the PRD I-like domain shared by both inducible elements, as well as the TG-like domain of IE-A4. This factor, distinct from the IRF-1, IRF-2 and the alpha F1 binding proteins and presenting a different affinity pattern from that of the TG protein, is proposed as a candidate for IFN-type I gene regulation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites / genetics
  • Cell Line
  • DNA-Binding Proteins / genetics*
  • Interferon-alpha / genetics*
  • Mice
  • Molecular Sequence Data
  • Newcastle disease virus
  • Positive Regulatory Domain I-Binding Factor 1
  • Promoter Regions, Genetic / genetics*
  • Regulatory Sequences, Nucleic Acid
  • Repressor Proteins / genetics*
  • Transcription Factors*
  • Transcription, Genetic*

Substances

  • DNA-Binding Proteins
  • Interferon-alpha
  • Prdm1 protein, mouse
  • Repressor Proteins
  • Transcription Factors
  • Positive Regulatory Domain I-Binding Factor 1