Abstract
A DNA-dependent DNA polymerase from rat liver mitochondria was partially purified and characterized. Mitochondrial DNA polymerase has been found to be quite different from other DNA-dependent DNA polymerases alpha and beta present in the rat liver in the following points: elution patterns in a DEAE-cellulose column chromatography, sedimentation coefficients determined by the glycerol gradient centrifugation in the presence of high salt, and sensitivities to N-ethylmaleimide, ethidium bromide and KCl.
MeSH terms
-
Animals
-
Calcium / pharmacology
-
DNA Polymerase I / isolation & purification
-
DNA Polymerase I / metabolism
-
DNA Polymerase II / isolation & purification
-
DNA Polymerase II / metabolism
-
DNA, Mitochondrial / biosynthesis*
-
DNA-Directed DNA Polymerase* / isolation & purification
-
DNA-Directed DNA Polymerase* / metabolism
-
Enzyme Activation
-
Kinetics
-
Magnesium / pharmacology
-
Manganese / pharmacology
-
Microscopy, Electron
-
Mitochondria, Liver / enzymology*
-
Mitochondria, Liver / ultrastructure
-
Molecular Weight
-
Rats
Substances
-
DNA, Mitochondrial
-
Manganese
-
DNA Polymerase I
-
DNA Polymerase II
-
DNA-Directed DNA Polymerase
-
Magnesium
-
Calcium