Differential requirements of CD45 for lymphocyte development and function

Eur J Immunol. 1995 Dec;25(12):3431-6. doi: 10.1002/eji.1830251234.


CD45 exon 6-deficient mice show a profound block of thymocyte development at a transitional differentiation step from immature CD4+ CD8+ to mature single-positive cells. Only a few T lymphocytes are observed in the periphery of such animals, but B cell development is not affected. We investigated whether mature B lymphocytes in CD45 exon 6-deficient mice have normal functions in the absence of CD45 expression and show here that CD45-defective B lymphocytes from CD45 exon 6-deficient mice have intact B lymphocyte functions, including T-dependent and T-independent antigen-specific antibody production and class switching in vivo if normal CD4+ T lymphocytes were adoptively transferred into the mutant mice. From these results, we conclude that CD45 expression on B lymphocytes is not essential for B cell responses following antigen stimulation in vivo. However, CD4+ T helper function was severely diminished in CD45 exon 6-deficient mice, suggesting that the requirements of CD45 molecules in antigen-specific response and development are different between T and B lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody-Producing Cells / cytology
  • B-Lymphocytes / classification
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / transplantation
  • Cell Differentiation / genetics
  • Cell Differentiation / immunology
  • Cell Division / drug effects
  • Cell Division / immunology
  • Epitopes
  • Immunophenotyping
  • Immunotherapy, Adoptive
  • Leukocyte Common Antigens / genetics
  • Leukocyte Common Antigens / physiology*
  • Lymphocyte Activation / drug effects
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout


  • Epitopes
  • Leukocyte Common Antigens