Interleukin-10 inhibits cytokine generation from mast cells

Eur J Immunol. 1996 Jan;26(1):166-70. doi: 10.1002/eji.1830260126.


This report examines the effects of recombinant murine interleukin-10 (rmIL-10) on antigen-induced beta-hexosaminidase, leukotriene (LT)C4 and cytokine release from mouse bone marrow-derived mast cells (BMMC). BMMC sensitized to hapten-monoclonal IgE directed against dinitrophenol-bovine serum albumin (DNP-BSA) and challenged with 10 ng/ml DNP-BSA generated beta-hexosaminidase and LTC4-like material which was followed by tumor necrosis factor-alpha (TNF-alpha) and granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA expression and protein release. Incubation of BMMC with 1-100 ng/ml rmIL-10 inhibited cytokine generation, without affecting beta-hexosaminidase and LTC4-like material release. TNF-alpha, but not GM-CSF mRNA expression, was also diminished in rmIL-10-treated BMMC, suggesting that down-regulation of cytokine production by rmIL-10 involves different mechanisms. These results identify a novel biological action of IL-10 as an inhibitor of cytokine production by stimulated mast cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells
  • Calcimycin / pharmacology
  • Cells, Cultured
  • Cytokines / antagonists & inhibitors*
  • Cytokines / biosynthesis*
  • Cytokines / genetics
  • Dinitrophenols / pharmacology
  • Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis
  • Granulocyte-Macrophage Colony-Stimulating Factor / genetics
  • Haptens / pharmacology
  • Interleukin-10 / pharmacology*
  • Mast Cells / drug effects*
  • Mast Cells / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Recombinant Proteins / pharmacology
  • Serum Albumin, Bovine / pharmacology
  • Tumor Necrosis Factor-alpha / biosynthesis
  • Tumor Necrosis Factor-alpha / genetics


  • Cytokines
  • Dinitrophenols
  • Haptens
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • dinitrophenyl-bovine serum albumin
  • Interleukin-10
  • Serum Albumin, Bovine
  • Calcimycin
  • Granulocyte-Macrophage Colony-Stimulating Factor