B cell-B cell interaction through intercellular adhesion molecule-1 and lymphocyte functional antigen-1 regulates immunoglobulin E synthesis by B cells stimulated with interleukin-4 and anti-CD40 antibody

Eur J Immunol. 1996 Jan;26(1):192-200. doi: 10.1002/eji.1830260130.


IgE synthesis by purified human B cells is induced by two signals: a class switching factor, most commonly interleukin (IL)-4, and the engagement of CD40, which is activated through its interaction with CD40 ligand (CD40L) expressed on activated T cells. Thus, the combination of IL-4 and anti-CD40 monoclonal antibody (mAb) has been shown to stimulate IgE production in vitro by highly purified B cells. In this T cell-independent system, strong homotypic aggregation of B cells is observed prior to the production of IgE. Flow cytometric analysis and cell binding assays showed that the stimulation of purified B cells with anti-CD40 mAb plus IL-4 resulted in a striking increase of intercellular adhesion molecule (ICAM)-1(CD54) expression, an induction of CD43 and an avidity change of lymphocyte functional antigen (LFA)-1(CD11a/CD18), with little augmentation of CD18 expression. Addition of anti-ICAM-1 mAb caused an inhibition of homotypic aggregation but augmented IgE synthesis by B cells stimulated with anti-CD40 mAb and IL-4, although it did not affect B cell proliferation or IL-6 production by the B cells. Among the mAb against counter-receptors for ICAM-1 tested, anti-CD11a mAb suppressed IgE synthesis, while anti-CD18 mAb and anti-CD43 mAb had little effect. The enhancing or inhibitory effect of anti-ICAM-1 mAb or anti-CD11a mAb on IgE production was achieved by the increased or decreased expression of germline C epsilon transcripts by B cells stimulated with anti-CD40 mAb and IL-4. These results indicate that B cell-B cell interaction through ICAM-1 and one of its counter receptors, LFA-1, regulates IgE synthesis by modulating C epsilon germ-line transcription.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / pharmacology*
  • Antigens, CD / immunology
  • B-Lymphocytes / metabolism*
  • CD18 Antigens / immunology
  • CD40 Antigens / immunology*
  • Cell Aggregation / immunology
  • Cell Communication / immunology*
  • Drug Synergism
  • Humans
  • Immunoglobulin Constant Regions / genetics
  • Immunoglobulin E / biosynthesis*
  • Immunoglobulin E / drug effects
  • Intercellular Adhesion Molecule-1 / physiology*
  • Interleukin-4 / pharmacology*
  • Leukosialin
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology
  • Lymphocyte Function-Associated Antigen-1 / physiology*
  • Sialoglycoproteins / immunology
  • Transcription, Genetic / immunology


  • Antibodies, Monoclonal
  • Antigens, CD
  • CD18 Antigens
  • CD40 Antigens
  • Immunoglobulin Constant Regions
  • Leukosialin
  • Lymphocyte Function-Associated Antigen-1
  • SPN protein, human
  • Sialoglycoproteins
  • Intercellular Adhesion Molecule-1
  • Interleukin-4
  • Immunoglobulin E