Molecular Cloning of a 74-kDa Regulatory Subunit (B" or Delta) of Human Protein Phosphatase 2A

FEBS Lett. 1996 Jan 22;379(1):107-11. doi: 10.1016/0014-5793(95)01500-0.

Abstract

Based on amino acid sequence data of a 74-kDa regulatory subunit (B" or delta) of a human heterotrimeric protein phosphatase 2A, a cDNA encoding the subunit was isolated from a human cerebral cortex library. The cDNA had an open reading frame encoding an M(r) 66,138 protein of 570 amino acids. Bacterial expression of the cDNA yielded a protein immunoreactive with antisera specific to the 74-kDa subunit. The predicted primary structure of the subunit had no similarity to already reported sequences of PP2A regulatory subunits including A, B, and PR72. Potential phosphorylation sites for protein kinases A and C, a bipartite motif of putative nuclear localization signal, and SH3 accessible proline-rich domain, and a unique PQ repeat were found in the sequence. The subunit mRNA of about 2.9 kb was ubiquitously expressed in rat tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cerebral Cortex / enzymology
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Escherichia coli / genetics
  • Humans
  • Male
  • Molecular Sequence Data
  • Molecular Weight
  • Phosphoprotein Phosphatases / chemistry*
  • Phosphoprotein Phosphatases / genetics*
  • Protein Conformation
  • Protein Phosphatase 2
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Repetitive Sequences, Nucleic Acid
  • Tissue Distribution

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 2

Associated data

  • GENBANK/AB000634
  • GENBANK/AB000635
  • GENBANK/D78360
  • GENBANK/L76702