3D solution structure of copper and silver-substituted yeast metallothioneins

FEBS Lett. 1996 Jan 22;379(1):85-93. doi: 10.1016/0014-5793(95)01492-6.


3D solution structural calculations for yeast silver(I)-substituted metallothionein (MT) and native copper(I) MT were completed using experimentally determined NOE and dihedral angle constraints, in conjunction with experimentally derived metal-to-Cys connectivities for AgMT which were assumed identical for CuMT. For the first 40 residues in both structures, the polypeptide backbone wraps around the metal cluster in two large parallel loops separated by a deep cleft containing the metal cluster. Minor differences between the two structures include differences in hydrogen bonds and the orientation of the N-terminus with the overall protein volume conserved to within 6.5%.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Copper / chemistry
  • Fungal Proteins / chemistry*
  • Fungal Proteins / genetics
  • Hydrogen Bonding
  • Magnetic Resonance Spectroscopy
  • Metallothionein / analogs & derivatives*
  • Metallothionein / chemistry*
  • Metallothionein / genetics
  • Models, Molecular
  • Molecular Sequence Data
  • Molecular Structure
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / genetics
  • Silver / chemistry
  • Solutions


  • Fungal Proteins
  • Solutions
  • Silver
  • Copper
  • Metallothionein