Translation of RNAIII, the Staphylococcus aureus agr regulatory RNA molecule, can be activated by a 3'-end deletion

FEMS Microbiol Lett. 1995 Nov 1;133(1-2):155-61. doi: 10.1111/j.1574-6968.1995.tb07877.x.

Abstract

RNAIII, an RNA molecule shown to encode delta-hemolysin and independently to regulate toxin synthesis in Staphylococcus aureus, is transcribed at the mid-exponential phase of growth, while its target genes are activated 2 h later, at the post-exponential phase of growth. We show here that the translation of RNAIII to the 26-amino acid peptide delta-hemolysin is delayed by 1 h, and that this delay is abolished when the 3'-end of this molecule is deleted. We suggest that structural changes of RNAIII to a translatable form of the molecule precede its regulation of target gene expression.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Toxins / genetics
  • Base Sequence
  • Cell Division / genetics
  • Gene Deletion
  • Gene Expression Regulation, Bacterial / genetics
  • Hemolysin Proteins / genetics
  • Molecular Sequence Data
  • Neurotoxins / genetics
  • Nucleic Acid Conformation
  • Protein Biosynthesis
  • RNA, Antisense / genetics*
  • RNA, Bacterial / genetics*
  • Staphylococcus aureus / cytology
  • Staphylococcus aureus / genetics*
  • Transcription, Genetic / genetics

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • Hemolysin Proteins
  • Neurotoxins
  • RNA, Antisense
  • RNA, Bacterial
  • RNAIII, Staphylococcus aureus
  • staphylococcal alpha-toxin
  • delta hemolysin protein, Staphylococcus aureus