Identification of alcA, a Bordetella bronchiseptica gene necessary for alcaligin production

Gene. 1995 Dec 29;167(1-2):133-6. doi: 10.1016/0378-1119(95)00659-1.

Abstract

The alcA gene, essential for the production of the dihydroxamate siderophore, alcaligin, by Bordetella bronchiseptica, was cloned and sequenced. The alcA gene was identified on a 4.7-kb EcoRI genomic fragment adjacent to a Tn5lac transposon insertion that inactivated alcaligin production in strain MBORD846. Analysis of the alcA nucleotide sequence revealed a putative Fur-binding site, suggesting that expression of this gene is repressed by iron. The deduced amino-acid sequence of this open reading frame had significant homology with the Escherichia coli iucD gene product, an enzyme required for biosynthesis of the dihydroxamate siderophore aerobactin.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Bordetella bronchiseptica / genetics*
  • Cloning, Molecular
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial
  • Hydroxamic Acids*
  • Iron / physiology
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Sequence Homology, Amino Acid
  • Siderophores / biosynthesis*

Substances

  • Hydroxamic Acids
  • RNA, Messenger
  • Siderophores
  • alcaligin
  • Iron

Associated data

  • GENBANK/U32117