Cloning and characterization of a Brassica napus gene encoding a homologue of the B subunit of a heteromeric CCAAT-binding factor

Gene. 1995 Dec 29;167(1-2):209-13. doi: 10.1016/0378-1119(95)00680-x.

Abstract

The CCAAT motif present in the promoter of several genes is recognized in yeast and animals by a highly specific heteromeric factor (variously called HAP, CBF, CP1 or NF-Y) which is composed of a minimum of three subunits. A plant homologue of the CBF-B/HAP2 subunit is described for the first time in this report. Sequence comparison of the Brassica napus (Bn) CCAAT-binding factor (CBF) B subunit with the homologous yeast and animal proteins revealed that the critical amino-acid domains involved in DNA binding and subunit assembly are also conserved in plants. Interestingly, the Gln-rich regions found in the animal and yeast proteins, which may be involved in transcriptional activation, are absent in the Bn CBF-B subunit. The analysis of various cDNAs and of a genomic clone revealed the presence of alternatively spliced transcripts which could originate from different promoters.

Publication types

  • Comparative Study

MeSH terms

  • Alternative Splicing
  • Base Sequence
  • Brassica / genetics*
  • CCAAT-Binding Factor*
  • CCAAT-Enhancer-Binding Proteins
  • Cloning, Molecular
  • DNA Primers / chemistry
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / genetics*
  • Fungal Proteins / genetics*
  • Genes, Plant
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics*

Substances

  • CCAAT-Binding Factor
  • CCAAT-Enhancer-Binding Proteins
  • DNA Primers
  • DNA, Complementary
  • DNA-Binding Proteins
  • Fungal Proteins
  • Transcription Factors

Associated data

  • GENBANK/S81259
  • GENBANK/S81261
  • GENBANK/U33884
  • GENBANK/U33885