Restricted Expression of galactose/N-acetylgalactosamine-specific Macrophage C-type Lectin to Connective Tissue and to Metastatic Lesions in Mouse Lung

Immunology. 1995 Dec;86(4):591-8.

Abstract

We investigated expression of macrophage galactose/N-acetylgalactosamine-specific C-type lectin (MMGL) in normal mouse lung tissue and in lungs with metastatic nodules produced by OV2944-HM-1 mouse metastatic ovarian tumour cells. Cells expressing MMGL were detected in tissue sections using a rat monoclonal antibody (mAB) specific for MMGL, mAb LOM-14. The regions containing cells immunostained using mAb LOM-14 were restricted to the connective tissue surrounding blood vessels and respiratory epithelia, whereas alveolar regions of lung parenchyma were essentially devoid of these cells. In contrast, a significant number of cells in the alveolar regions was shown to express Mac-1 antigen (CD11b/CD18) and leucocyte common antigen (CD45). Immunoelectron microscopic study revealed the presence of MMGL in the intracellular vesicles of cells residing in connective tissue. In the tumour-bearing mice, MMGL-positive cells were also present within metastatic nodules. Their localization outside of the nodules was restricted to connective tissue. Cells with Mac-1 antigens were seen both in the nodules and in the alveolar regions. These results indicate that MMGL serves as a unique macrophage marker in mouse lung tissue due to its topographical site-dependent pattern of expression. The present results also suggest a possible involvement of macrophages expressing MMGL in the immune response directed against metastatic tumour cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Asialoglycoproteins
  • Carrier Proteins / metabolism*
  • Connective Tissue / metabolism
  • Immunoenzyme Techniques
  • Lectins / metabolism*
  • Lectins, C-Type*
  • Lung / metabolism*
  • Lung / ultrastructure
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / secondary*
  • Macrophages, Alveolar / metabolism*
  • Membrane Proteins*
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Microscopy, Immunoelectron
  • Neoplasm Transplantation

Substances

  • Antibodies, Monoclonal
  • Asialoglycoproteins
  • Carrier Proteins
  • Clec10a protein, mouse
  • Clec10a protein, rat
  • Lectins
  • Lectins, C-Type
  • Membrane Proteins