Frequent development of murine T-cell lymphomas with TcR alpha/beta+, CD4-/8- phenotype after implantation of human inflammatory breast cancer cells in BALB/c nude mice

Jpn J Cancer Res. 1995 Nov;86(11):1086-96. doi: 10.1111/j.1349-7006.1995.tb03025.x.

Abstract

Tumors developed quite frequently in some of the visceral organs, including spleen and liver, in BALB/c nude mice upon subcutaneously xenografting surgical specimens from five different inflammatory breast cancer patients. All of these tumors developed within two and a half months to one year after the subcutaneous inoculation of surgical specimens. From these tumors, five independent transplantable tumors, including tMK-2, tHK-1, tYK-1, tYK-2 and tTY-1 have been established. Chromosome analysis, morphologic studies by light and electron microscopy and phenotype analysis indicated that these tumors are of mouse origin. The tMK-2 tumor was highly metastatic to the spleen and liver when it was subcutaneously transplanted into the right scapular region. In addition, the region where the tMK-2 tumor cells were subcutaneously inoculated showed an apparently inflammatory process represented by erythema. After subcutaneous inoculation into the right scapular region, tHK-1, tYK-1, 2, and tTY-1 tumors also metastasized to some of the visceral organs, including spleen and liver. From these tumors, in vitro cell lines were established. The cells grew in a stromal-cell dependent manner under in vitro culture conditions. The cells were again tumorigenic at the inoculated region and metastasized to various organs, including liver and spleen, of BABL/c nude mice. Histological examination revealed that the tumors showed features of malignant lymphoma. Phenotypically, these five tumors expressed early T lymphocyte markers as revealed by anti-mouse anti-TcR alpha/beta, anti-CD3, CD4 and CD8 monoclonal antibodies. To our knowledge, these cell lines are the first T-cell lines showing the phenotype of extrathymically differentiated T-cells in the liver.

MeSH terms

  • Animals
  • Antigens, Neoplasm / analysis*
  • Breast Neoplasms / immunology
  • Breast Neoplasms / pathology*
  • CD4 Antigens / analysis
  • CD8 Antigens / analysis
  • Carcinoma / immunology
  • Carcinoma / pathology*
  • Cell Transformation, Neoplastic
  • Cells, Cultured
  • Coculture Techniques
  • Connective Tissue / pathology
  • DNA, Neoplasm / analysis
  • Erythema / etiology
  • Female
  • Humans
  • Immunophenotyping
  • Inflammation
  • Injections, Subcutaneous
  • Karyotyping
  • Liver / pathology
  • Liver Neoplasms / pathology
  • Lymphoma, T-Cell / etiology*
  • Lymphoma, T-Cell / genetics
  • Lymphoma, T-Cell / pathology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Neoplasm Metastasis
  • Neoplasm Transplantation / adverse effects*
  • Neoplasm Transplantation / immunology
  • Receptors, Antigen, T-Cell, alpha-beta / analysis
  • Species Specificity
  • Spleen / pathology
  • Splenic Neoplasms / pathology
  • Transplantation, Heterologous / adverse effects*
  • Transplantation, Heterologous / immunology
  • Tumor Cells, Cultured / pathology
  • Tumor Cells, Cultured / transplantation

Substances

  • Antigens, Neoplasm
  • CD4 Antigens
  • CD8 Antigens
  • DNA, Neoplasm
  • Receptors, Antigen, T-Cell, alpha-beta