Molecular heterogeneity of Acinetobacter baumanii isolates during seasonal increase in prevalence

Infect Control Hosp Epidemiol. 1995 Oct;16(10):590-4. doi: 10.1086/647013.

Abstract

Objective: To study the epidemiology of Acinetobacter baumanii during a period of increased seasonal prevalence.

Design: Descriptive molecular and clinical epidemiologic study of A baumanii isolates from 1990 through 1992.

Setting: A 770-bed urban, acute, tertiary-care university hospital.

Results: During 1990 through 1992, the rate of A baumanii isolations was 30.4 per 1,000 culture isolations during the summer, compared to 12.6 per 1,000 culture isolations during the fall, winter, and spring (P < .000001). There were 320 isolates identified among 260 patients during this time. Eighty-one patients with isolates available were identified for evaluation; they ranged in age from 2 months to 95 years. Pulsed-field gel electrophoresis (PFGE) typing showed that 59 (83%) of 71 typed isolates had distinct PFGE patterns. There were three small clusters of isolates with the same PFGE patterns, suggesting cross-transmission in those instances.

Conclusions: A seasonally increased prevalence of A baumanii largely associated with device-related nosocomial infections in severely ill patients was noted over a 3-year period. Although there were isolated instances of cross-transmission, most isolates had distinct PFGE patterns. Clonal dissemination of a single strain was not responsible for the seasonal increased prevalence of A baumanii. PFGE typing was useful in directing control efforts.

Publication types

  • Comparative Study

MeSH terms

  • Acinetobacter / classification
  • Acinetobacter / genetics
  • Acinetobacter / isolation & purification*
  • Acinetobacter Infections / epidemiology*
  • Acinetobacter Infections / microbiology
  • Adolescent
  • Adult
  • Aged
  • Child
  • Child, Preschool
  • Connecticut / epidemiology
  • Cross Infection / epidemiology*
  • Cross Infection / microbiology
  • DNA, Bacterial / genetics
  • Electrophoresis, Gel, Pulsed-Field
  • Genetic Heterogeneity
  • Hospitals, University
  • Humans
  • Infant
  • Microbial Sensitivity Tests
  • Middle Aged
  • Ohio / epidemiology
  • Prevalence
  • Seasons*

Substances

  • DNA, Bacterial