In vitro screening of traditional medicines for anti-hypertensive effect based on inhibition of the angiotensin converting enzyme (ACE)

K Hansen; U Nyman; U W Smitt; A Adsersen; L Gudiksen; S Rajasekharan; P Pushpangadan

doi:10.1016/0378-8741(95)01286-m

In vitro screening of traditional medicines for anti-hypertensive effect based on inhibition of the angiotensin converting enzyme (ACE)

J Ethnopharmacol. 1995 Aug 11;48(1):43-51. doi: 10.1016/0378-8741(95)01286-m.

Authors

K Hansen¹, U Nyman, U W Smitt, A Adsersen, L Gudiksen, S Rajasekharan, P Pushpangadan

Affiliation

¹ Department of Pharmacognosy, Royal Danish School of Pharmacy, Copenhagen, Denmark.

PMID: 8569246
DOI: 10.1016/0378-8741(95)01286-m

Abstract

Traditional medicines reported to be used as anti-hypertensives or diuretics from different regions in the world (China, India and South America) have been investigated. The bioassay is based on inhibition of ACE, as measured from the enzymatic cleavage of the chromophore-fluorophore-labelled substrate dansyltriglycine into dansylglycine and diglycine. In total, 31 species have been investigated and the crude extracts from seven species inhibit the enzyme by more than 50%.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Angiotensin-Converting Enzyme Inhibitors / pharmacology*
Antihypertensive Agents / pharmacology*
Chromatography, High Pressure Liquid
Dansyl Compounds / analysis
Drug Evaluation, Preclinical
Medicine, Traditional*
Plant Extracts / analysis
Plant Extracts / pharmacology
Plants, Medicinal / chemistry*

Substances

Angiotensin-Converting Enzyme Inhibitors
Antihypertensive Agents
Dansyl Compounds
Plant Extracts