Effects of cisplatin on cell cycle kinetics, morphological change, and cleavage pattern of DNA in two human ovarian carcinoma cell lines

Oncology. Jan-Feb 1996;53(1):19-26. doi: 10.1159/000227529.

Abstract

We evaluated the effects of cisplatin on the cell kinetics, cytomorphological changes, and cleavage patterns of DNA in two lines of human ovarian carcinoma cells. KF-1 cells displayed a cell cycle arrest in the G2M phase, while HMG cells displayed a transient cell accumulation in the S phase, without obvious G2M arrest. Morphological changes characterized by condensation and fragmentation of chromatin, and DNA cleavage by oligonucleosome-sized DNA fragments were observed in the HMG cells but not in the KF-1 cells. The pattern of cell death in HMG cells was considered to be apoptosis, but that of KF-1 cells necrosis. These findings showed the different mechanisms of anti-tumor effect of cisplatin on human ovarian carcinoma cell lines, including cell kinetics and pattern of cell death.

MeSH terms

  • Carcinoma / pathology*
  • Cell Cycle / drug effects*
  • Cell Size / drug effects
  • Cell Survival / drug effects
  • Cisplatin / administration & dosage*
  • Cycloheximide / administration & dosage
  • DNA Damage / drug effects*
  • Female
  • Humans
  • Ovarian Neoplasms / pathology*
  • Tumor Cells, Cultured

Substances

  • Cycloheximide
  • Cisplatin