Fusion of the EWS gene to CHN, a member of the steroid/thyroid receptor gene superfamily, in a human myxoid chondrosarcoma

Oncogene. 1996 Jan 18;12(2):229-35.

Abstract

The specific chromosomal translocation t(9;22)(q22-31;q11-12) has been observed in the myxoid variant of human chondrosarcoma. In agreement with this observation we report that the EWS gene located at chromosome band 22q12 becomes fused to CHN, a member of the steroid/thyroid receptor gene superfamily located at 9q22-31, in a skeletal myxoid chondrosarcoma. CHN appears to be the human homologue of the rat gene NOR1, which was recently identified as a sequence overexpressed in rat brain cells undergoing apoptosis. Our results also indicate that the chimaeric EWS-CHN gene encodes a EWS-CHN fusion protein in which the C-terminal RNA-binding domain of EWS is replaced by the entire CHN protein, comprising a long N-terminal domain, a central DNA binding domain and a C-terminal ligand-binding/dimerisation domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chondrosarcoma / genetics*
  • Cloning, Molecular
  • DNA / metabolism
  • Humans
  • Molecular Sequence Data
  • Oncogenes*
  • Polymerase Chain Reaction
  • Rats
  • Receptors, Steroid / genetics*
  • Receptors, Thyroid Hormone / genetics*
  • Sarcoma, Ewing / genetics*

Substances

  • Receptors, Steroid
  • Receptors, Thyroid Hormone
  • DNA

Associated data

  • GENBANK/S81242
  • GENBANK/S81243