Development and application of an antibody ELISA for the marker protein of ovine pulmonary carcinoma

Vet Immunol Immunopathol. 1995 Aug;47(3-4):323-31. doi: 10.1016/0165-2427(94)05409-l.


Ovine pulmonary carcinoma (OPC) is a contagious pulmonary neoplasia with a suspected retroviral etiology. The major core protein (P27) of the putative OPC virus cross-reacts with antibodies to P27 of the Mason-Pfizer monkey virus (MPMV), a type-D retrovirus. This serological reactivity serves as the only accepted biological marker for OPC. In order to make a useful reagent for the detection of the OPC marker for serodiagnosis and epidemiological studies, the MPMV-P27 coding region was cloned and expressed in Escherichia coli. Gel purified recombinant MPMV-P27 protein was used to develop an immunoassay. This recombinant enzyme-linked immunosorbent assay (ELISA) was then used to screen 223 sera from US sheep and 176 sera from Italian sheep. In this study, we found: (1) a high prevalence of infection with the putative OPC retrovirus in sheep with chronic pneumonia; (2) a subclinical infection with OPC virus may be more common in US sheep than indicated by the rare recorded occurrence of pulmonary carcinoma; (3) an apparent association between ovine lentivirus (OLV) and OPC infection.

MeSH terms

  • Adenocarcinoma, Bronchiolo-Alveolar / diagnosis
  • Adenocarcinoma, Bronchiolo-Alveolar / veterinary*
  • Adenocarcinoma, Bronchiolo-Alveolar / virology
  • Animals
  • Antibodies, Viral / analysis
  • Base Sequence
  • Biomarkers, Tumor
  • Blotting, Western
  • DNA Primers / chemistry
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Escherichia coli / genetics
  • Gene Expression Regulation, Viral
  • Lung Neoplasms / diagnosis
  • Lung Neoplasms / veterinary*
  • Lung Neoplasms / virology
  • Macaca
  • Mason-Pfizer monkey virus / genetics
  • Mason-Pfizer monkey virus / immunology
  • Molecular Sequence Data
  • Prevalence
  • Recombinant Proteins / immunology
  • Retroviridae Infections / diagnosis
  • Retroviridae Infections / veterinary*
  • Retroviridae Infections / virology
  • Sensitivity and Specificity
  • Sheep
  • Sheep Diseases / diagnosis*
  • Sheep Diseases / virology
  • Tumor Virus Infections / diagnosis
  • Tumor Virus Infections / veterinary*
  • Tumor Virus Infections / virology
  • Viral Core Proteins / genetics
  • Viral Core Proteins / immunology*
  • Visna-maedi virus / genetics
  • Visna-maedi virus / immunology


  • Antibodies, Viral
  • Biomarkers, Tumor
  • DNA Primers
  • Recombinant Proteins
  • Viral Core Proteins