Reverse transcriptase from avian myeloblastosis virus

J Virol. 1979 Feb;29(2):517-22. doi: 10.1128/JVI.29.2.517-522.1979.

Abstract

From lots of 20 to 30 g of avian myeloblastosis virus RNA-dependent DNA polymerase was obtained in preparations of purity greater than 95% by using a two-step column chromatographic procedure employing DEAE (DE 52) and carboxymethylcellulose (CM 52.). Yields of RNA-dependent DNA polymerase varied from approximately 20,000 to 35,000 U/g of virus. Specific activity of the enzyme was about 35,000 to 60,000 U/mg of protein. Free of detectable RNase activity, the product exhibited a molecular weight of about 160,000, an isoelectric point of 6.5, and approximately 2 mol of fatty acid per mol of enzyme.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Avian Leukosis / microbiology*
  • Avian Leukosis Virus / enzymology*
  • Avian Myeloblastosis Virus / enzymology*
  • Blood / microbiology
  • Chickens
  • Fatty Acids / analysis
  • Glycoproteins / analysis
  • Isoelectric Point
  • Molecular Weight
  • RNA-Directed DNA Polymerase* / analysis
  • RNA-Directed DNA Polymerase* / isolation & purification
  • RNA-Directed DNA Polymerase* / metabolism
  • Viral Proteins / analysis

Substances

  • Fatty Acids
  • Glycoproteins
  • Viral Proteins
  • RNA-Directed DNA Polymerase