Hexavalent-chromium reduction by a chromate-resistant Bacillus sp. strain

Antonie Van Leeuwenhoek. 1995 Oct;68(3):203-8. doi: 10.1007/BF00871816.


Bacillus strain QC1-2, isolated from a chromium-polluted zone, was selected by its high ability to both tolerate and reduce hexavalent chromium [Cr(VI)] to less-toxic trivalent chromium [Cr(III)]. Cell suspensions of strain QC1-2 rapidly reduced Cr(VI), in both aerobic and anaerobic conditions, to Cr(III) which remained in the supernatant. Cr(VI) reduction was dependent on the addition of glucose but sulfate, an inhibitor of chromate transport, had no effect. Studies with permeabilized cells and cell extracts showed that the Cr(VI) reductase of strain QC1-2 is a soluble NADH-dependent enzyme.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerobiosis
  • Anaerobiosis
  • Bacillus / drug effects
  • Bacillus / metabolism*
  • Bacillus subtilis / drug effects
  • Bacillus subtilis / metabolism
  • Bacterial Proteins / metabolism*
  • Cell Membrane Permeability
  • Chromates / metabolism*
  • Chromates / pharmacology
  • Drug Resistance, Microbial
  • Glucose / metabolism
  • Oxidation-Reduction
  • Oxidoreductases / metabolism*
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / metabolism
  • Sulfates / pharmacology


  • Bacterial Proteins
  • Chromates
  • Sulfates
  • Oxidoreductases
  • chromate reductase
  • Glucose