Identification of the S-Endo 1 endothelial-associated antigen

Biochem Biophys Res Commun. 1996 Jan 5;218(1):210-6. doi: 10.1006/bbrc.1996.0037.

Abstract

We have recently described a monoclonal antibody, S-Endo 1, recognizing a molecule constitutively expressed in all types of human endothelial cells. We showed that this protein around 118 kDa and located at the endothelial cell-cell junction presented sequence identity with MUC18 described as a tumor marker in human melanoma. The difference in antibodies immunoreactivity and antigen molecular weight heterogeneity observed between various cell types strongly suggested S-Endo 1 antigen isoforms expression.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antigens, CD*
  • Antigens, Surface / analysis*
  • Antigens, Surface / chemistry
  • Blotting, Western
  • CD146 Antigen
  • Cell Line
  • Cells, Cultured
  • Cross Reactions
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism*
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Humans
  • Immunoenzyme Techniques
  • Membrane Glycoproteins / analysis
  • Membrane Glycoproteins / chemistry
  • Molecular Sequence Data
  • Neural Cell Adhesion Molecules*
  • Sequence Homology, Amino Acid
  • Umbilical Veins

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Surface
  • CD146 Antigen
  • MCAM protein, human
  • Membrane Glycoproteins
  • Neural Cell Adhesion Molecules