PCR-based identification of wheat genomes

Mol Ecol. 1995 Aug;4(4):509-14. doi: 10.1111/j.1365-294x.1995.tb00246.x.

Abstract

We describe a PCR system that distinguishes the A, B and D genomes in wheat DNA extracts. PCRs were directed at the 'non-transcribed spacer' regions of the rDNA loci. The spacers within the D genome locus have a 71-bp insertion that is absent from the corresponding A and B loci. PCR product sizes therefore enable D- and D+ genomes to be distinguished. The A and B genomes can be differentiated by PCR with an internal primer which does not anneal to A genome sequences. This work is relevant to the ancient ecology of wheat, as it is often difficult to determine ploidy level from morphological examination of archaeobotanical remains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • DNA Primers
  • DNA, Plant / genetics*
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Genetics, Population
  • Genome, Plant*
  • In Situ Hybridization
  • Molecular Sequence Data
  • Polymerase Chain Reaction*
  • Polyploidy
  • Transcription, Genetic
  • Triticum / genetics*

Substances

  • DNA Primers
  • DNA, Plant
  • DNA, Ribosomal