Block of P-type Ca2+ channels in freshly dissociated rat cerebellar Purkinje neurons by diltiazem and verapamil

Brain Res. 1995 Oct 9;695(1):88-91. doi: 10.1016/0006-8993(95)00815-8.


We investigated the effects of organic Ca2+ channel blockers, diltiazem and verapamil, on the high voltage-activated P-type Ca2+ channels in freshly isolated rat Purkinje neurons. Both diltiazem and verapamil blocked P-type Ca2+ channel current without any change in the current-voltage relation. The block was concentration-dependent. In the presence of these agents, the inactivation curve was shifted to hyperpolarizing potentials. The characteristics of block of P-type Ca2+ channels by diltiazem and verapamil are similar to that of L-type Ca2+ channels. These results indicate that both benzothiazepine and phenylalkylamine react with P-type Ca2+ channels and suggest that some structural features common to which operate in both L-type and P-type Ca2+ channels may be involved in drug binding to these channels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium Channels / drug effects*
  • Cerebellum / drug effects*
  • Diltiazem / pharmacology*
  • Dose-Response Relationship, Drug
  • Purkinje Cells / drug effects
  • Rats
  • Rats, Wistar
  • Time Factors
  • Verapamil / pharmacology*


  • Calcium Channels
  • Verapamil
  • Diltiazem