Mouse Eotaxin expression parallels eosinophil accumulation during lung allergic inflammation but it is not restricted to a Th2-type response

Immunity. 1996 Jan;4(1):1-14. doi: 10.1016/s1074-7613(00)80293-9.

Abstract

A model of lung eosinophilia based on the repeated exposure of mice to aerosolized OVA has been used to identify C-C chemokine genes expressed at stages of massive eosinophil infiltration. We describe the identification and cloning of a cDNA that encodes a mouse C-C chemokine with 68% amino acid identity to guinea pig Eotaxin. The recombinant protein encoded by this gene displays potent and specific chemotactic activity for eosinophils, both in vivo and in vitro. Its mRNA levels parallel the kinetics of eosinophil accumulation in the lung during the experimentally induced eosinophilia and it is mainly produced by type I alveolar epithelial cells. The mRNA expression of mouse Eotaxin is not restricted to Th2 T cells in vitro and is independent of the development of a Th2-type response during N. brasiliensis infection, in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Movement
  • Chemokine CCL11
  • Chemokines, CC*
  • Cloning, Molecular
  • Cytokines / biosynthesis*
  • Cytokines / genetics
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Eosinophils / immunology*
  • Eosinophils / pathology
  • Female
  • Inflammation / immunology
  • Inflammation / pathology
  • Lung Diseases / immunology*
  • Lung Diseases / pathology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • RNA, Messenger / analysis*
  • Respiratory Hypersensitivity / immunology
  • Sequence Alignment
  • Th2 Cells / immunology*
  • Th2 Cells / pathology

Substances

  • Ccl11 protein, mouse
  • Chemokine CCL11
  • Chemokines, CC
  • Cytokines
  • DNA, Complementary
  • RNA, Messenger

Associated data

  • GENBANK/U40672