IL-5-deficient mice have a developmental defect in CD5+ B-1 cells and lack eosinophilia but have normal antibody and cytotoxic T cell responses

Immunity. 1996 Jan;4(1):15-24. doi: 10.1016/s1074-7613(00)80294-0.


Mice deficient in interleukin-5 (IL-5-/- mice) were generated by gene targeting in embryonal stem cells. Contrary to previous studies, no obligatory role for IL-5 was demonstrated in the regulation of conventional B (B-2) cells, in normal T cell-dependent antibody responses or in cytotoxic T cell development. However, CD5+ B cells (B-1 cells) in the peritoneal cavity were reduced by 50%-80% in 2-week-old IL-5-/- mice, returning to normal by 6-8 weeks of age. The IL-5-/- mice did not develop blood and tissue eosinophilia when infected with the helminth Mesocestoides corti, but basal levels of eosinophils with normal morphology were produced in the absence of IL-5. IL-5 deficiency did not affect the worm burden of infected mice, indicating that increased eosinophils do not play a significant role in the host defence in this parasite model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Formation
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / pathology
  • CD5 Antigens / biosynthesis*
  • Cell Differentiation
  • Embryonic and Fetal Development / immunology*
  • Eosinophilia / genetics
  • Eosinophilia / immunology*
  • Eosinophilia / parasitology
  • Interleukin-5 / deficiency*
  • Mesocestoides
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • T-Lymphocytes / immunology*


  • CD5 Antigens
  • Interleukin-5