Isolation of a Chlamydomonas reinhardtii telomere by functional complementation in yeast

Curr Genet. 1995 Oct;28(5):437-40. doi: 10.1007/BF00310812.

Abstract

We attempted to determine whether Chlamydomonas reinhardtii telomeres, which do not form G-quartet structures readily in vitro, are able to nucleate telomere addition in Saccharomyces cerevisiae. Restricted C. reinhardtii genomic DNA was ligated to a linear S. cerevisiae vector lacking a telomere. A C. reinhardtii telomere ligated to this unprotected end allowed vector replication as a linear DNA molecule in S. cerevisiae. DNA sequencing revealed common [T4AG3]n and variant T6AG3 and T5AG3 C. reinhardtii telomere repeats capped by S. cerevisiae telomere repeat units. The recognition of a C. reinhardtii telomere by the telomere maintenance machinery of S. cerevisiae is consistent with a common theme for telomere structure in organisms with divergent telomere repeats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chlamydomonas reinhardtii / genetics*
  • Cloning, Molecular
  • DNA, Recombinant
  • Escherichia coli / genetics
  • Genetic Complementation Test*
  • Molecular Sequence Data
  • Saccharomyces cerevisiae / genetics*
  • Telomere*

Substances

  • DNA, Recombinant

Associated data

  • GENBANK/X77590